Adherent Cell Culture

Esco Aster offers clients the service to develop cell expansion and scale-up manufacture of human mesenchymal stromal cells (MSCs) isolated from various sources, regardless of development status.

Parameters such as hMSC seeding density, growth duration, harvesting parameters, and media exchange intervals would be optimised with emphasis on goals to reduce costs, increase efficiency, and maximise yield whilst not compromising hMSC quality.

Metabolite and media analysis, including but not limited to glucose, pH change, cellular waste products, as well as cell morphology monitoring via fluorescence imaging are utilised to ascertain the ideal process conditions and parameters.

Expanded cells can be screened for various quality release criteria respective to the cells, such as the ‘Minimal criteria for defining multipotent mesenchymal stromal cells (MSCs)’ established by the International Society of Cellular and Gene Therapy (ISCT) for expanded MSCs, or pluripotent markers, and ability to differentiate into 3 germ layers (ecto-, endo- and mesoderm) such as in the case for induced pluripotent stem cells.

In addition, the option for usage of xeno-free or GMP certified cell culture media and reagents paves the pathway towards clinical manufacturing and regulatory approval of expanded cells.