Gene Therapy

Overview

Gene Therapy is the use of genetic material to manipulate a patient's cells for the treatment of an inherited or acquired disease.

The genetic material can be delivered directly as DNA or DNA complexes (non-viral methods) or packaged within a viral vector (recombinant virus) to efficiently taken up by appropriate cells. There are different viruses that can be used for human gene therapy as viral vectors, including retrovirus, adenovirus, lentivirus, herpes simplex, vaccinia and adeno-associated virus.

Esco Aster works with a variety of viral vector such as Lentivirus (LV), Retrovirus (RV) and Adeno-Associated virus (AAV), providing an end-to-end production of these viral vectors.

With the use of Esco Aster in-house bioreactors, viral vectors can be produced at a scalable and affordable rate.

Role of Viral Vectors:

Viral vectors are commonly used in the field of gene therapy to deliver desired gene into the cells. The use of viral vector was adopted due to its efficiency in infecting cells. Due to the increasing number of leads in the field, there bound to have a high demand for these viral vectors for varies clinical trials. Thus, there will be a bottleneck in the viral vector production.

Viral Vector Production Services

Efficient, large-scale production of lentivirus, retrovirus, adenovirus-associated, adenovirus stocks compared to cell factories and other 2D culture systems using producer cell lines such as:

  • HEK-293T
  • Phoenix-GP
  • PG13

Key Application:
CAR-T Cell Therapy /
CAR-Immune Cell Therapy

CAR-T cell therapy / CAR-Immune Cell Therapy is an immunotherapy to treat cancer. T cells/ Immune cells are re-engineered with the help of viral vector to expression surface receptors that can specifically recognize and target cancer cells.

Esco Aster’s Viral Vector Production Workflow:
Single-step Transient Transfection

Single-step Transient Transfection

Esco Aster’s Viral Vector Production Workflow:
Dual-step Transient Transfection

Dual-step Transient Transfection