Animal Health Services

Overview

The seamless functioning of our planet relies heavily on the immense contributions of animals. They hold a crucial position in maintaining environmental balance, sustaining ecosystems, and impacting our daily lives. Therefore, prioritizing their well-being becomes paramount. 

The illnesses that afflict animals have a direct impact on human health as well. By preventing and managing diseases, we not only foster economic growth and support local communities, but also enhance the health of susceptible groups including our children and seniors. 

Esco Aster understands the importance of prioritizing animal health and well-being. Embracing translational research using the One Health approach, we are dedicated to ensuring the vitality of animals in various aspects. Our CRDMO capabilities encompass vaccines, stem cells and exosomes extending to animal proteins, agriculture, and the bioindustry.

VACCINES

Nurturing animals’ lives through proactive vaccination practices

The benefits of vaccines have long been understood by generations of parents who’ve protected the health of their children. Similarly, for farmers, veterinarians and pet owners, vaccines provide essential protections against illness in livestock and pets.

Shielding the animals against zoonotic diseases not only preserves their health but also prevents transmission to humans. Therefore, the positive impact of vaccination not only benefits them, but also extends to the public health as well.

Esco Aster Vaccine Production Capabilities

With an aim of ensuring a reliability of vaccine supply and immunization to support the growing livestock and poultry industry, our proprietary Tide Motion® technology offers the means of achieving high-cell density working cell bank cultures to support the scalable production of veterinary vaccines.

Our Vaccine Production Workflow

The Tide Motion® manufacturing platform offers a smooth and seamless vaccine production workflow.

In addition, a modularly integrated system with our Cell Processing Isolator (CPI), helps to localize vaccine production making it more affordable - in terms of CAPEX & OPEX - for developing countries by ensuring a smooth bioprocessing workflow.

Adherent Cell Lines Constituting Our Animal Vaccine Portfolio

Cell lines are permanently established culture of animal cells that can be propagated repeatedly. Their usage is the most convenient method for virus cultivation in the laboratory which are to be utilized in different applications. 

Our animal vaccine portfolio comprises of the following adherent cell lines:

  • VERO: Peste des Petits Ruminants (PPR) Virus, Porcine Epidemic Diarrhea Virus (PEDV) and Rift Valley Fever (RVF) Virus
  • BHK-21: Animal Rabies
  • MARC-145: Porcine Reproductive and Respiratory Syndrome Virus (PRRSV)
  • ST: Swine Vaccines (e.g. Hog Cholera, PEDV)
  • CEF Primary Lines: Marek’s Virus
  • Lamb Testis Cell Line: Lumpy Skin Disease (LSD) Virus

Fish Vaccines

During the past 20 years, fish vaccines have become an established, proven, and cost-effective method of controlling certain infectious diseases in aquaculture worldwide. Fish vaccines can significantly reduce specific disease-related losses resulting in a reduction of antibiotic use. 

Fish vaccines are advantageous over antibiotics because they are natural biological materials that leave no residue in the product or the environment, and therefore will not induce a resistant strain of the disease organism.

At Esco Aster, we help collaborators in providing autogenous vaccines tailored for specific disease and farm.

LEARN MORE

Fish Vaccines Technologies

Fish Vaccine Technologies

Capabilities for Autogenous Vaccines

Cell and virus banking for specific pathogenic strains
Process optimization and production of autogenous vaccines
QC testing for batch release (in-vitro tests)

High Viral Titer with TideXcell™ Bioreactor

Culture conditions tend to be variable for different cell lines and very high cell densities; hence vaccines with a high titer which are capable of eliciting good immune responses have been achieved in TideXcell™ bioreactors.

As a consequence of high cell densities and high viral titers in the range of 8 log10 frequently the Tide Motion® bioreactors are able to effectively replace static culture systems as shown below:

Swine Vaccine

Vaccine2D Culture system
(No. of units)
Tide Motion® Bioreactor
(No. of units)
Hog cholera
Roller bottles (2500cm2) > 123
TideXcell™ - 002 (1)
PEDVT150-flasks > 285CelCradle™ - 500A (1)
PRRSVT175 - flasks > 132CelCrade™ - 500A (1)

H5N1 and H1N1

VaccineRoller Bottle
(850 cm2, 2L)
TideXcell™
(10L)
H5N11000-20001
H1N110001

Rabbies Vaccine

CelCradle™ - 500ARoller BottlesRoux Bottles
Media1000 ml4550 ml4550 ml
Fetal Calf Serum100 ml455 ml455 ml
Culture System12613
Filtration
-YY
Washing
-YY
Packaging-YY
Sterilization-YY
Virus Titre106-7LD50/ml
106LD50/ml106LD50/ml

Peste des Petits Ruminants

Equivalence
Titer of virus obtained from vs CelCradle™
32 roller bottles=1 CelCradle-500 A
(Volume = 8L)

Titer = 7 X TCID50 per ml

(Volume = 2.5L dilutted to 8L)

Titer = 7.167 TCID50 per ml

Animal Health in Cell and Gene Therapy Applications

In the past decade, the potential to translate scientific discoveries in the area of regenerative therapeutics in veterinary species to novel, effective human therapies has gained interest from the scientific and public domains. Innovative cell and gene therapies are an area of rapid development that can prevent and ameliorate pain and suffering in companion animals and humans alike.

Mesenchymal Stromal Cells

The most well-studied cell type in veterinary medicine, ex-vivo expanded mesenchymal stromal cells (MSCs)  are adherent cells that have regenerative and immunomodulatory properties through their paracrine activity. 

The most common and successful veterinary use of MSCs treats osteoarthritis in dogs, as well as injuries to bones, joints, tendons, ligaments, and the spinal cord. Early research also shows that MSCs might successfully treat dry eye in dogs and stomatitis a severe, painful oral disease in cats.

As a CRDMO, Esco Aster’s proprietary technology utilizing Tide Motion bioreactors is ideal in cultivating billions of anchorage dependent cells like MSCs. Our technology saves space, utilities and labor requirements due to its compact and automated design especially when compared to roller bottles or multi-layered culture wares.

Induced Pluripotent Stem Cells (iPSCs)

Induced pluripotent stem cells (iPSCs) are pluripotent cells that can be obtained by reprogramming animal and human differentiated cells. They are widely used in therapeutics for disease modeling, regenerative medicine, and drug discovery. 

As of today, there has been an accumulation of iPSC researches for domestic animals (e.g. porcine, equine, canine, feline, bovine, etc.). Aside from  their benefit in treating veterinary pathologies, porcine, equine and canine models have been shown to be valuable for the study and treatment of human diseases as well. 

With Esco Aster’s deep expertise in iPSC reprogramming, we offer iPSC differentiation services utilizing our streamed-line protocols and proprietary Tide Motion® technologies. 

Specialized Immune Cells (CAR-T)

First approved by the USFDA in 2017 to treat B-cell acute lymphoblastic leukemia and diffuse large B-cell lymphoma, CAR-T cell therapy is one of most promising new treatments for cancer. It uses the patient’s T cells modified by gene therapy to express a recombinant receptor that allows targeted cytolysis.

In relation with animal health, current studies suggest that canine cancers like B cell lymphoma complements preclinical models in testing and optimizing the safety and efficacy of these therapies including feasibility, toxicity profiles, immune correlates, and outcomes providing new treatment options for canine patients.

At Esco Aster, we leverage our expertise into the production of genetically-modified cell therapy such as CAR-T cells. Our adaptable and dynamic approaches employed across multiple cell types enable us to provide process design and developmental solutions all the way through manufacturing and supply. 

Exosomes/Extracellular Vesicles

Exosomes represent a potentially accurate and sensitive  biomarkers, both in diagnosing and monitoring variety of diseases in humans and animals. Extracellular vesicles (EVs) are secreted by most cell types, and their function is in facilitating intracellular communication. Exosomes in both humans and veterinary medicine find their major application in neoplastic diseases.

Esco Aster’s patented and proprietary Tide Motion® technology is used for the continuous production of exosomes or EVs. This system is equipped with different process modes for ease of feed of fresh culture medium and harvest of secreted product. The harvested medium is clarified via filtration to remove cell debris and residual media components, post which, it is concentrated, and buffer exchanged to desirable media via tangential flow filtration (TFF). Purified EVs is formulated into a medical naïve EV product or be loaded with a specific cargo.

References :

  1. Gene Therapy in Dogs - Conditions Treated, Procedure, Efficacy, Recovery, Cost, Considerations, Prevention. (n.d.). Gene Therapy in Dogs - Conditions Treated, Procedure, Efficacy, Recovery, Cost, Considerations, Prevention. https://wagwalking.com/treatment/gene-therapy
  2. Ma J, Bruce TJ, Jones EM, Cain KD. A Review of Fish Vaccine Development Strategies: Conventional Methods and Modern Biotechnological Approaches. Microorganisms. 2019; 7(11):569. https://doi.org/10.3390/microorganisms7110569
  3. Optimization of canine CD20 chimeric antigen receptor T cell manufacturing and in vitro cytotoxic activity against B-cell lymphoma - PubMed. (2020, December 1). PubMed. https://doi.org/10.1111/vco.12602
  4. Scarfone, R. A., Pena, S. M., Russell, K. A., Betts, D. H., & Koch, T. G. (2020, December 8). The use of induced pluripotent stem cells in domestic animals: a narrative review - BMC Veterinary Research. BioMed Central. https://doi.org/10.1186/s12917-020-02696-7